Recombinant Mouse Klrk1 protein, His-tagged

• Cat.No.: Klrk1-5643M
• Product Overview: Recombinant Mouse Klrk1 protein(O54709-1)(Phe90-Val232), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Phe90-Val232
• Tag: N-His
• Form: Lyophilized from 0.22μm filtered solution in 20mM Tris, 250mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse NKG2D, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human MICA, hFc Tag with the EC50 of 71.3ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 17.5 kDa. Due to glycosylation, the protein migrates to 24-35kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Klrk1 killer cell lectin-like receptor subfamily K, member 1 [ Mus musculus ]
• Official Symbol: Klrk1
• Synonyms: KLRK1; killer cell lectin-like receptor subfamily K, member 1; NKG2-D type II integral membrane protein; NK cell receptor D; natural killer cell group 2D; NKG2-D-activating NK receptor; Nkg2d; NKG2-D; D6H12S2489E
• Gene ID: 27007
• mRNA Refseq: NM_001083322
• Protein Refseq: NP_001076791