Recombinant Human Keratin 14, His-tagged

• Cat.No.: KRT14-467H
• Product Overview: Recombinant human KRT14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. MW: 53.8 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-472aa
• Description: KRT14 is a member of the cytokeratin subfamily of intermediate filament proteins (IFPs). It is unique in that it is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. It functions to regulate cell growth and size through its interactions with the adaptor protein 14-3-3 sigma to mediate protein synthesis.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.4M Urea.
• Molecular Mass: 53.8 kDa (492aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MTTCSRQFTS SSSMKGSCGI GGGIGGGSSR ISSVLAGGSC RAPSTYGGGL SVSSSRFSSG GAYGLGGGYG GGFSSSSSSF GSGFGGGYGG GLGTGLGGGF GGGFAGGDGL LVGSEKVTMQ NLNDRLASYL DKVRALEEAN ADLEVKIRDW YQRQRPAEIK DYSPYFKTIE DLRNKILTAT VDNANVLLQI DNARLAADDF RTKYETELNL RMSVEADING LRRVLDELTL ARADLEMQIE SLKEELAYLK KNHEEEMNAL RGQVGGDVNV EMDAAPGVDL SRILNEMRDQ YEKMAEKNRK DAEEWFFTKT EELNREVATN SELVQSGKSE ISELRRTMQN LEIELQSQLS MKASLENSLE ETKGRYCMQL AQIQEMIGSV EEQLAQLRCE MEQQNQEYKI LLDVKTRLEQ EIATYRRLLE GEDAHLSSSQ FSSGSQSSRD VTSSSRQIRT KVMDVHDGKV VSTHEQVLRT KN
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1.0 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: KRT14 keratin 14 [ Homo sapiens ]
• Official Symbol: KRT14
• Synonyms: KRT14; keratin 14; EBS3, EBS4, keratin 14 (epidermolysis bullosa simplex, Dowling Meara, Koebner); keratin, type I cytoskeletal 14; epidermolysis bullosa simplex; Dowling Meara; Koebner; CK-14; keratin-14; cytokeratin 14; cytokeratin-14; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner); K14; NFJ; CK14; EBS3; EBS4
• Gene ID: 3861
• mRNA Refseq: NM_000526
• Protein Refseq: NP_000517
• MIM: 148066
• UniProt ID: P02533
• Chromosome Location: 17q12-q21
• Pathway: Glucocorticoid receptor regulatory network, organism-specific biosystem
• Function: protein binding; structural constituent of cytoskeleton