Recombinant Human KDM5B protein, His-tagged

• Cat.No.: KDM5B-3798H
• Product Overview: Recombinant Human KDM5B protein(190-309 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 190-309 aa
• AA Sequence: SLRCLQKPNLTTDTKDKEYKPHDIPQRQSVQPSETCPPARRAKRMRAEAMNIKIEPEETTEARTHNLRRRMGCPTPKCENEKEMKSSIKQEPIERKDYIVENEKEKPKSRSKKATNAVDL
• Purity: 90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: KDM5B lysine (K)-specific demethylase 5B [ Homo sapiens ]
• Official Symbol: KDM5B
• Synonyms: KDM5B; lysine (K)-specific demethylase 5B; JARID1B, jumonji, AT rich interactive domain 1B , Jumonji, AT rich interactive domain 1B (RBP2 like); lysine-specific demethylase 5B; cancer/testis antigen 31; CT31; PLU 1; RBBP2H1A; RBP2-H1; histone demethylase JARID1B; putative DNA/chromatin binding motif; jumonji, AT rich interactive domain 1B; jumonji/ARID domain-containing protein 1B; retinoblastoma-binding protein 2 homolog 1; retinoblastoma-binding protein 2, homolog 1A; PLU1; PUT1; PLU-1; JARID1B; FLJ10538; FLJ12459; FLJ12491; FLJ16281; FLJ23670
• Gene ID: 10765
• mRNA Refseq: NM_006618
• Protein Refseq: NP_006609
• MIM: 605393
• UniProt ID: Q9UGL1