Recombinant Human KAT2B, His-tagged

• Cat.No.: KAT2B-8483H
• Product Overview: Recombinant Human KAT2B, fused with His tag, was expressed in Sf21 Cells.

Specification

• Description: PCAF belongs to the GCN5/PCAF family of nuclear HATs. The proteins consist of N-terminal and C-terminal domains separated by a deep hydrophobic cleft. The C-terminus contains the HAT domain and a bromodomain, while the N-terminus contains a PCAF-specific domain. CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. Numerous studies indicate that these HATs function as histone- acetylating transcriptional co-activators . Besides histones, PCAF also acetylates non-histone proteins. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.
• Source: Sf21 Cells
• Species: Human
• Tag: His
• Form: 20 mM Tris-Cl, pH 7.9, 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
• AA Sequence: MSEAGGAGPG GCGAGAGAGA GPGALPPQPA ALPPAPPQGS PCAAAAGGSG ACGPATAVAA AGTAEGPGGG GSARIAVKKA QLRSAPRAKK LEKLGVYSAC KAEESCKCNG WKNPNPSPTP PRADLQQIIV SLTESCRSCS HALAAHVSHL ENVSEEEMNR LLGIVLDVEY LFTCVHKEED ADTKQVYFYL FKLLRKSILQ RGKPVVEGSL EKKPPFEKPS IEQGVNNFVQ YKFSHLPAKE RQTIVELAKM FLNRINYWHL EAPSQRRLRS PNDDISGYKE NYTRWLCYCN VPQFCDSLPR YETTQVFGRT LLRSVFTVMR RQLLEQARQE KDKLPLEKRT LILTHFPKFL SMLEEEVYSQ NSPIWDQDFL SASSRTSQLG IQTVINPPPV AGTISYNSTS SSLEQPNAGS SSPACKASSG LEANPGEKRK MTDSHVLEEA KKPRVMGDIP MELINEVMST ITDPAAMLGP ETNFLSAHSA RDEAARLEER RGVIEFHVVG NSLNQKPNKK ILMWLVGLQN VFSHQLPRMP KEYITRLVFD PKHKTLALIK DGRVIGGICF RMFPSQGFTE IVFCAVTSNE QVKGYGTHLM NHLKEYHIKH DILNFLTYAD EYAIGYFKKQ GFSKEIKIPK TKYVGYIKDY EGATLMGCEL NPRIPYTEFS VIIKKQKEII KKLIERKQAQ IRKVYPGLSC FKDGVRQIPI ESIPGIRETG WKPSGKEKSK EPRDPDQLYS TLKSILQQVK SHQSAWPFME PVKRTEAPGY YEVIRFPMDL KTMSERLKNR YYVSKKLFMA DLQRVFTNCK EYNPPESEYY KCANILEKFF FSKIKEAGLI DK
• Purity: >95% as determined by SDS-PAGE
• Storage: -80 °C
• Shipping: Dry Ice

Gene Information

• Gene Name: KAT2B K(lysine) acetyltransferase 2B [ Homo sapiens ]
• Official Symbol: KAT2B
• Synonyms: KAT2B; K(lysine) acetyltransferase 2B; p300/CBP associated factor , PCAF; histone acetyltransferase KAT2B; GCN5; GCN5L; P/CAF; histone acetylase PCAF; CREBBP-associated factor; p300/CBP-associated factor; lysine acetyltransferase 2B; histone acetyltransferase PCAF; P; CAF; PCAF
• Gene ID: 8850
• mRNA Refseq: NM_003884
• Protein Refseq: NP_003875
• MIM: 602303
• UniProt ID: Q92831
• Chromosome Location: 3p24
• Pathway: Androgen Receptor Signaling Pathway, organism-specific biosystem; Delta-Notch Signaling Pathway, organism-specific biosystem; E2F transcription factor network, organism-specific biosystem; FoxO family signaling, organism-specific biosystem; Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; HTLV-I infection, organism-specific biosystem
• Function: acetyltransferase activity; cyclin-dependent protein kinase inhibitor activity; histone acetyltransferase activity; histone deacetylase binding; lysine N-acetyltransferase activity; lysine N-acetyltransferase activity; protein binding; protein kinase binding; transcription coactivator activity; transcription cofactor activity; transcription factor binding; transferase activity, transferring acyl groups