Recombinant Human KAT2B, GST-tagged

• Cat.No.: KAT2B-163H
• Product Overview: Recombinant Human PCAF bromodomain (714-831 aa) fused to a GST tag at the N-terminus

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: 714-831 a.a.
• Description: The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. p300/CBP-associated factor (PCAF) is a transcriptional coactivator that works both as a histone lysine acetyltransferase, through its HAT domain, and as an acetyl-lysine reader through its conserved bromodomain located directly C-terminal to the HAT domain. The PCAF bromodomain binds acetylated histone H3 and H4 as well as non-histone targets. Bromodomain binding is dictated by the position of the acetylated lysine as well as interactions with specific residues flanking the acetyl-lysine. PCAF also specifically binds the HIV viral protein Tat on acetylated K50 to regulate its transactivating activity and help induce chromatin remodeling of proviral genes, thereby promoting transcription of viral proteins.
• Form: Liquid. 50 mM Tris, pH 8.0, containing 150 mM sodium chloride and 20% glycerol.
• Molecular Mass: 40.9 kDa (714-831 aa + NT GST Tag)
• Purity: ≥95%
• Storage: Store at -80°C. Avoid repeated freeze and thaw cycles. Stable for 1 year.

Gene Information

• Gene Name: KAT2B K(lysine) acetyltransferase 2B [ Homo sapiens ]
• Official Symbol: KAT2B
• Synonyms: KAT2B; K(lysine) acetyltransferase 2B; p300/CBP associated factor , PCAF; histone acetyltransferase KAT2B; GCN5; GCN5L; P/CAF; histone acetylase PCAF; CREBBP-associated factor; p300/CBP-associated factor; lysine acetyltransferase 2B; histone acetyltransferase PCAF; P; CAF; PCAF
• Gene ID: 8850
• mRNA Refseq: NM_003884
• Protein Refseq: NP_003875
• MIM: 602303
• UniProt ID: Q92831
• Chromosome Location: 3p24
• Pathway: Androgen Receptor Signaling Pathway, organism-specific biosystem; Delta-Notch Signaling Pathway, organism-specific biosystem; E2F transcription factor network, organism-specific biosystem; FoxO family signaling, organism-specific biosystem; Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; HTLV-I infection, organism-specific biosystem
• Function: acetyltransferase activity; cyclin-dependent protein kinase inhibitor activity; histone acetyltransferase activity; histone deacetylase binding; lysine N-acetyltransferase activity; lysine N-acetyltransferase activity; protein binding; protein kinase binding; transcription coactivator activity; transcription cofactor activity; transcription factor binding; transferase activity, transferring acyl groups