Recombinant Human Karyopherin (importin) Beta 1, His-tagged

• Cat.No.: KPNB1-1316H
• Product Overview: Recombinant human KPNB1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: KPNB1 is a member of the importin beta family. This protein is involved in nuclear protein import, either by associating itself with an adapter protein (for example, importin-alpha subunit which binds to nuclear localization signals (NLS) in cargo substrates), or by acting autonomously as a nuclear transport receptor (serves as NLS receptor, docking of the importin/substrate complex to the nuclear pore complex).
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 30% glycerol, 0.1 NaCl.
• Molecular Weight: 99.6 kDa (899 aa)
• Purity: > 85 % by SDS-PAGE
• Concentration: 0.25 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGSMELITIL EKTVSPDRLE LEAAQKFLER AAVENLPTFL VELSRVLANP GNSQVARVAA GLQIKNSLTS KDPDIKAQYQ QRWLAIDANA RREVKNYVLQ TLGTETYRPS SASQCVAGIA CAEIPVNQWP ELIPQLVANV TNPNSTEHMK ESTLEAIGYI CQDIDPEQLQ DKSNEILTAI IQGMRKEEPS NNVKLAATNA LLNSLEFTKA NFDKESERHF IMQVVCEATQ CPDTRVRVAA LQNLVKIMSL YYQYMETYMG PALFAITIEA MKSDIDEVAL QGIEFWSNVC DEEMDLAIEA SEAAEQGRPP EHTSKFYAKG ALQYLVPILT QTLTKQDEND DDDDWNPCKA AGVCLMLLAT CCEDDIVPHV LPFIKEHIKN PDWRYRDAAV MAFGCILEGP EPSQLKPLVI QAMPTLIELM KDPSVVVRDT AAWTVGRICE LLPEAAINDV YLAPLLQCLI EGLSAEPRVA SNVCWAFSSL AEAAYEAADV ADDQEEPATY CLSSSFELIV QKLLETTDRP DGHQNNLRSS AYESLMEIVK NSAKDCYPAV QKTTLVIMER LQQVLQMESH IQSTSDRIQF NDLQSLLCAT LQNVLRKVQH QDALQISDVV MASLLRMFQS TAGSGGVQED ALMAVSTLVE VLGGEFLKYM EAFKPFLGIG LKNYAEYQVC LAAVGLVGDL CRALQSNIIP FCDEVMQLLL ENLGNENVHR SVKPQILSVF GDIALAIGGE FKKYLEVVLN TLQQASQAQV DKSDYDMVDY LNELRESCLE AYTGIVQGLK GDQENVHPDV MLVQPRVEFI LSFIDHIAGD EDHTDGVVAC AAGLIGDLCT AFGKDVLKLV EARPMIHELL TEGRRSKTNK AKTLATWATK ELRKLKNQA
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Activation of DNA fragmentation factor; Apoptosis; Apoptosis induced DNA fragmentation; Apoptotic execution phase; HIV Infection; Host Interactions of HIV factors; Influenza Infection; Influenza Life Cycle; Interactions of Rev with host cellular proteins; Nuclear import of Rev protein; RNA transport; RNA transport; Role of Calcineurin-dependent NFAT signaling in lymphocytes; Signaling events mediated by TCPTP; TGF-beta Receptor Signaling Pathway

Gene Information

• Gene Name: KPNB1 karyopherin (importin) beta 1 [ Homo sapiens ]
• Official Symbol: KPNB1
• Synonyms: KPNB1; karyopherin (importin) beta 1; IMB1; IPO1; IPOB; Impnb; NTF97; MGC2155; MGC2156; MGC2157; karyopherin beta 1; importin 1; importin 90; nuclear factor p97; importin beta-1 subunit; Importin-90; Karyopherin subunit beta-1; Nuclear factor P97; karyopherin (importin) beta 1
• Gene ID: 3837
• mRNA Refseq: NM_002265
• Protein Refseq: NP_002256
• MIM: 602738
• UniProt ID: Q14974
• Chromosome Location: 17q21.32
• Function: nuclear localization sequence binding; protein binding; protein domain specific binding; protein transporter activity; zinc ion binding