Recombinant Human JAG2 protein, His-tagged

• Cat.No.: JAG2-1869H
• Product Overview: Recombinant Human JAG2 aa. (Phe714~His981) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: The Notch signaling pathway is an intercellular signaling mechanism that is essential for proper embryonic development. Members of the Notch gene family encode transmembrane receptors that are critical for various cell fate decisions. The protein encoded by this gene is one of several ligands that activate Notch and related receptors. Two transcript variants encoding different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Phe714~His981
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >92%
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Isoelectric Point: 6.6

Gene Information

• Gene Name: JAG2 jagged 2 [ Homo sapiens (human) ]
• Official Symbol: JAG2
• Synonyms: JAG2; jagged 2; HJ2; SER2; protein jagged-2
• Gene ID: 3714
• mRNA Refseq: NM_002226.4
• Protein Refseq: NP_002217.3
• UniProt ID: Q9Y219