Recombinant Human ITPA, His-tagged

• Cat.No.: ITPA-778H
• Product Overview: Recombinant human ITPA protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW =23.7kDa (215aa) confirmed by MALDI-TOF.

Specification

• Cat. No.: ITPA-778H
• Description: ITPA, also known as Inosine triphosphate pyrophosphatase, is an enzyme that catalyzes the pyrophosphohydrolysis of both ITP (inosine triphosphate) and dITP (deoxyinosine triphosphate) to IMP (inosine monophosphate) and diphosphate. IMP can be used as a substrate for purine nucleotide pathways. IMP can be phosphorylated to ITP, and ITPA can regulate the concentration of ITP in the cell by converting ITP back to IMP. Defects in ITPA result in ITPA deficiency which is thought to be inherited and is characterized by an over-accumulation of ITP in erythocytes, leukocytes and fibroblasts.
• Source: E.coli.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MMAASLVGKK IVFVTGNAKK LEEVVQILGD KFPCTLVAQK IDLPEYQGEP DEISIQKCQE AVRQVQGPVLVEDTCLCFNA LGGLPGPYIK WFLEKLKPEG LHQLLAGFED KSAYALCTFA LSTGDPSQPV RLFRGRTSGR IVAPRGCQDF GWDPCFQPDG YEQTYAEMPK AEKNAVSHRF RALLELQEYF GSLAA
• Purity: > 95% by SDS – PAGE.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: ITPA inosine triphosphatase (nucleoside triphosphate pyrophosphatase) [ Homo sapiens ]
• Synonyms: ITPA; inosine triphosphatase (nucleoside triphosphate pyrophosphatase); ITPase; C20orf37; dJ794I6.3; HLC14-06-P; inosine triphosphate pyrophosphatase; My049 protein; inosine triphosphatase-A; putative oncogene protein HLC14-06-P; nucleoside triphosphate diphosphatase; inosine triphosphate pyrophosphohydrolase; EC 3.6.1.19
• Gene ID: 3704
• mRNA Refseq: NM_033453
• Protein Refseq: NP_258412
• MIM: 147520
• UniProt ID: Q9BY32
• Chromosome Location: 20p
• Pathway: Drug metabolism - other enzymes; Metabolic pathways; Purine metabolism; Pyrimidine metabolism
• Function: hydrolase activity; metal ion binding; nucleoside-triphosphate diphosphatase activity