Recombinant Human IRS1 Protein, His-tagged

• Cat.No.: IRS1-2338H
• Product Overview: Recombinant Human IRS1 Protein (Asp12-Phe264) with a N-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Asp12-Phe264
• Description: This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 31.6 kDa
  Accurate Molecular Mass: 32 kDa
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: IRS1 insulin receptor substrate 1 [ Homo sapiens (human) ]
• Official Symbol: IRS1
• Synonyms: IRS1; insulin receptor substrate 1; HIRS 1; IRS-1; HIRS-1
• Gene ID: 3667
• mRNA Refseq: NM_005544
• Protein Refseq: NP_005535
• MIM: 147545
• UniProt ID: P35568