Recombinant Human IRF5 293 Cell Lysate

• Cat.No.: IRF5-5163HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for interferon regulatory factor 5 (IRF5), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: IRF5 interferon regulatory factor 5 [ Homo sapiens ]
• Official Symbol: IRF5
• Synonyms: IRF5; interferon regulatory factor 5; IRF-5; SLEB10
• Gene ID: 3663
• mRNA Refseq: NM_002200
• Protein Refseq: NP_002191
• MIM: 607218
• UniProt ID: Q13568
• Chromosome Location: 7q32
• Pathway: Apoptosis, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; Direct p53 effectors, organism-specific biosystem; Immune System, organism-specific biosystem; Interferon Signaling, organism-specific biosystem; Interferon alpha/beta signaling, organism-specific biosystem; Interferon gamma signaling, organism-specific biosystem
• Function: protein binding; regulatory region DNA binding; sequence-specific DNA binding transcription factor activity