Recombinant Human Interleukin 18, His-tagged

• Cat.No.: IL18-536H
• Product Overview: Recombinant human IL18 was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Human
• Tag: His
• Description: Interleukin 18 (IL-18), previously known as interferon-gamma (IFN-Y)-inducing factor, is an activator of NK cells and a co-inducer of Th1 cytokines. IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IL-18 was expressed in E. coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
• Concentration: 1 mg/ml
• Form: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, containing 10% glycerol.
• Purity: > 90% by SDS - PAGE
• Sequence: 36-192 amino acids: MGSSHHHHHH SSGLVPRGSH MNFGRLHCTT AVIRNINDQV LFVDKRQPVF EDMTDIDQSA SEPQTRLIIY MYKDSEVRGL AVTLSVKDSK MSTLSCKNKI ISFEEMDPPE NIDDIQSDLI FFQKRVPGHN KMEFESSLYE GHFLACQKED DAFKLILKKK DENGDKSVMF TLTNLHQS
• Molecular Mass: 20.4 kDa (178 aa), confirmed by MALDI-TOF.
• Applications: SDS-PAGE
• Endotoxin Levels: < 1.0 EU per ug of protein (determined by LAL method)
• Storage: Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: IL18 interleukin 18 (interferon-gamma-inducing factor) [ Homo sapiens ]
• Official Symbol: IL18
• Synonyms: IL18; IGIF; IL-18; IL-1g; IL1F4; MGC12320; interleukin 18 (interferon-gamma-inducing factor); interleukin-1 gamma; IFN-gamma-inducing factor
• Gene ID: 3606
• mRNA Refseq: NM_001562
• Protein Refseq: NP_001553
• MIM: 600953
• UniProt ID: Q14116
• Chromosome Location: 11q22.2-q22.3
• Pathway: African trypanosomiasis; Cellular roles of Anthrax toxin; Cytokine Signaling in Immune system; Cytokine-cytokine receptor interaction; Cytosolic DNA-sensing pathway; Immune System
• Function: Cytokine activity; signal transducer activity

Q&As

What are the difference between IL18-01HG, IL18-153HG and IL18-500H. (sequence, biological activity, QC, or what do you propose to buy)

IL18-01HG: Sequence: a.a.37-193 Endotoxin: <20 EU/mg Bio-activity: >5.0 x 10^4 IU/mg QC: Activity test, SDS-PAGE, LAL, SEC-HPLC IL18-153HG: Protein length: Tyr37-Asp193 AA Sequence: YFGKLESKLS VIRNLNDQVL FIDQGNRPLF EDMTDSDCRD NAPRTIFIISMYKDSQPRGM AVTISVKCEK ISTLSCENKI ISFKEMNPPD NIKDTKSDIIFFQRSVPGHD NKMQFESSSY EGYFLACEKE RDLFKLILKK EDELGDRSIMFTVQNEDVD Bio-activity: 1.0*10^6IU Measured by its ability to induce IFN-gamma secretion by KG‐1 human acute myelogenous leukemia cells in the presence of TNF-alpha. QC: Activity test, SDS-PAGE, LAL IL18-500H Recombinant Human IL18 will be custom produced upon order. All the sequence, purity, activity, and QC procedures will be optional and decided by our customer.