Recombinant Human Interferon, Gamma, HQ-tagged

• Cat.No.: IFNG-685H
• Product Overview: Recombinant human IFNGcontains 143 amino acid residues and a 13 amino acid Histidine-based tag fora total length of 156 aminoacids. The predicted molecular weight of therecombinant IFNG with His tag polypeptide is 16.6 kDa, but the recombinantprotein migrates as two bands with an apparent molecular mass of 25 and 27kDa in SDS-PAGE as a result of glycosylation.

Specification

• Cat. No.: IFNG-685H
• Description: IFNG is a dimerized soluble cytokine that is theonly member of the type II class of interferons. This interferon wasoriginally called macrophage-activating factor, a term now used to describe alarger family of proteins to which IFN-γ belongs. In humans, the IFN-γprotein is encoded by the IFNG gene.
• Source: Hordeum vulgare(barley grain)
• Species: Human
• Form: Lyophilized from a0.2 pm sterile filtered solution in PBS, pH 7.4.
• Purity: >98% asdetermined by SDS-PAGE analysis. Purified product carries no pyrogenic orpro-inflammatory contaminants, as assayed with monocyte activation test usingHuman 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1 betainduction.
• Endotoxin Level: <0.005 ng/ug ofIFN-gamma
• Biological Activity: Determined by thedose-dependent cytostatic effects of serial dilutions of IFN-gamma in thepresence of TNF-alpha, on HT-29 (human carcinoma) cells. The EDSO istypically below 5 ng/ml.
• Amino Acid Sequence: 2.00 x 105units/mg
• Reconstitution: Centrifuge vialprior to opening. Add sterile PBS to the vial to fully solubilize the proteinto a concentration not less than 100 ug/ml.
• Storage: Store lyophilizedprotein at -20°C and reconstituted protein in working aliquots at -20°C witha carrier protein (0.1%HSA or BSA) as a stabilizer for long term storage. Pleasenote that the addition of any carrier protein into this product may produceunwanted endotoxin. This depends upon the particular application employed.Avoid repeated freeze-thaw cycles.
• OfficialSymbol: IFNG
• Tag: Non

Gene Information

• Gene Name: IFNG interferon, gamma [ Homosapiens ]
• Synonyms: IFNG; interferon,gamma; IFG; IFI; interferon gamma; IFN-gamma;immune interferon; OTTHUMP00000240111
• Gene ID: 3458
• mRNA Refseq: NM_000619
• Protein Refseq: NP_000610
• MIM: 147570
• UniProt ID: P01579
• Chromosome Location: 12q14
• Pathway: ATF-2transcription factor network; Africantrypanosomiasis; Allograft rejection;Amoebiasis; Antigen processing and presentation; Cytokine Signaling in Immune system; Graft-versus-host disease; Herpessimplex infection; Hypertrophy Model;IFN-gamma pathway; IL2-mediated signaling events; Immune System; InfluenzaA; Interferon Signaling; Leishmaniasis; Measles
• Function: cytokineactivity; interferon-gamma receptor binding