Recombinant Human IFNG protein, His-tagged

Cat.No. : IFNG-480H
Product Overview : Recombinant Human IFNG (P01579) aa. (Gln24~Gln166) fused with His tag was produced in HEK293F cells.
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Description : This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases.
Source : HEK293F
Species : Human
Tag : His
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 18.4kDa; Accurate Molecular Mass: 22&25kDa as determined by SDS-PAGE reducing conditions.
Protein length : Gln24~Gln166
Endotoxin : <1.0EU per 1ug (determined by the LAL method)
Purity : > 95%
Characteristic : Isoelectric point is 9.7.
Applications : SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays; Purification; Amine Reactive Labeling
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Concentration : 200ug/mL
Storage buffer : 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300.
Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex
Gene Name IFNG interferon gamma [ Homo sapiens (human) ]
Official Symbol IFNG
Synonyms IFNG; interferon gamma; IFG; IFI; IFN-gamma; immune interferon
Gene ID 3458
mRNA Refseq NM_000619.2
Protein Refseq NP_000610.2
UniProt ID P01579

Not For Human Consumption!

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