Recombinant Human inhibitor of DNA binding 1, dominant negative helix-loop-helix protein, His-tagged

• Cat.No.: ID1-531H
• Product Overview: ID1, 1-155aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-155 a.a.
• Description: ID1 is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Recombinant human ID1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Molecular Mass: 18.5 kDa (178aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMKVASGS TATAAAGPSC ALKAGKTASG AGEVVRCLSE QSVAISRCAG GAGARLPALL DEQQVNVLLY DMNGCYSRLK ELVPTLPQNR KVSKVEILQH VIDYIRDLQL ELNSESEVGT PGGRGLPVRA PLSTLNGEIS ALTAEAACVP ADDRILCR
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: ID1 inhibitor of DNA binding 1, dominant negative helix-loop-helix protein [ Homo sapiens ]
• Official Symbol: ID1
• Synonyms: ID1; inhibitor of DNA binding 1, dominant negative helix-loop-helix protein; DNA-binding protein inhibitor ID-1; bHLHb24; dJ857M17.1.2; DNA binding protein inhibitor ID 1; inhibitor of differentiation 1; class B basic helix-loop-helix protein 24; dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein); ID
• Gene ID: 3397
• mRNA Refseq: NM_002165
• Protein Refseq: NP_002156
• MIM: 600349
• UniProt ID: P41134
• Chromosome Location: 20q11
• Pathway: ALK1 signaling events, organism-specific biosystem; IL-3 Signaling Pathway, organism-specific biosystem; Id Signaling Pathway, organism-specific biosystem; Notch-mediated HES/HEY network, organism-specific biosystem; TGF-beta signaling pathway, organism-specific biosystem; TGF-beta signaling pathway, conserved biosystem
• Function: protein binding