Recombinant Human IL4R & IL2RG protein(Met1-His232 & Met1-Asn254), His-tagged

• Cat.No.: IL4R & IL2RG-1061H
• Product Overview: Recombinant Human IL4R (NP_000409.1)(Met1-His232) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant Human IL2RG (P31785-1) (Met1-Asn254) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the IL4R & IL2RG was purified in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• ProteinLength: Met1-His232 & Met1-Asn254
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant heterodimer of human IL4R & IL2RG comprises 452 (209+243) amino acids and has a calculated molecular mass of 52.74 (23.91+28.83) KDa. The apparent molecular mass of human IL4R & IL2RG is approximately 47.81 KDa respectively in SDS-PAGE.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL4R interleukin 4 receptor [ Homo sapiens ]
• Official Symbol: IL4R
• Synonyms: IL4R; interleukin 4 receptor; interleukin-4 receptor subunit alpha; CD124; IL-4 receptor subunit alpha; interleukin-4 receptor alpha chain; IL4RA; IL-4RA
• Gene ID: 3566
• mRNA Refseq: NM_000418
• Protein Refseq: NP_000409
• MIM: 147781
• UniProt ID: P24394