Recombinant Human IL33 Protein, His-tagged

• Cat.No.: IL33-212H
• Product Overview: Recombinant Human Interleukin-33 is produced by our E.coli expression system and the target gene encoding Ser112-Thr270 is expressed with a 6His tag at the N-terminus.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Interleukin-33 (IL-33) belongs to the IL-1 superfamily. IL33 is highly expressed in endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes, but almost undetectable in placenta. IL33 induces the production of T helper-2 (Th2)-associated cytokines. IL-33 is a cytokine that mediates its biological effects by binding to and signals through IL1RL1/ST2 and IL-1 Receptor Accessory Protein (IL1RAP), activating intracellular molecules in the NF-κB and MAP kinase signaling pathways that drive production of type 2 cytokines from polarized Th2 cells.
• Form: Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, 1mM DTT, pH 7.25.
• Molecular Mass: Predicted Molecular Weight: 20.3kDa
  Apparent Molecular Weight: 21kDa, reducing conditions.
• AA Sequence: Ser112-Thr270
• Endotoxin: Less than 0.1 ng/ug (1 EU/ug).
• Purity: >95%
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days. Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months.
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting
• Reconstitution: It is not recommended to reconstitute to a concentration less than 100μg/ml.
  Dissolve the lyophilized protein in distilled water.
  Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Quality Statement: Purity: greater than 95% as determined by reducing SDS-PAGE.
• Shipping: The product is shipped at ambient temperature.
  Upon receipt, store it immediately at the temperature listed below.

Gene Information

• Gene Name: IL33 interleukin 33 [ Homo sapiens (human) ]
• Official Symbol: IL33
• Synonyms: Interleukin-33; IL-33; Interleukin-1 Family Member 11; IL-1F11; Nuclear Factor From High Endothelial Venules; NF-HEV; IL33; C9orf26; IL1F11; NFHEV; DVS27; IL1F11; NFEHEV
• Gene ID: 90865
• mRNA Refseq: NM_001199640.2
• Protein Refseq: NP_001186569.1
• MIM: 608678
• UniProt ID: O95760