Recombinant Human IL33, His-tagged

• Cat.No.: IL33-322H
• Product Overview: Total 169 AA. MW: 19.2 kDa (calculated). N-Terminal His-tag, 10 extra AA (highlighted).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: IL33 is a cytokine belonging to the IL-1 superfamily. IL33 induces helper T cells, mast cells, eosinophils and basophils to produce type 2 cytokines. This cytokine was previously named NF-HEV "nuclear factor (NF) in high endothelial venules, (HEVs) since it was originally identified in these specialized cells. IL-33 mediates its biological effects by interacting with the receptors ST2 (aka IL1RL1) and IL-1 Receptor Accessory Protein (IL1RAP), activating intracellular molecules in the NF-μB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-5 and IL-13) from polarized Th2 cells. The induction of type 2 cytokines by IL33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL33.
• Form: Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20 mM TRIS, 50 mM NaCl, Ph 7.5
• Research topic: Energy metabolism and body weight regulation
• Purity: >95%
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein.
• Sequences of amino acids: MKHHHHHHAS SITGISPITE YLASLSTYND QSITFALEDE SYEIYVEDLK KDEKKDKVLL SYYESQHPSN ESGDGVDGKM LMVTLSPTKD FWLHANNKEH SVELHKCEKP LPDQAFFVLH NMHSNCVSFE CKTDPGVFIG VKDNHLALIK VDSSENLCTE NILFKLSET
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

Gene Information

• Gene Name: IL33 interleukin 33 [ Homo sapiens ]
• Official Symbol: IL33
• Synonyms: IL33; interleukin 33; DVS27; NF-HEV; NFEHEV; C9orf26; DKFZp586H0523; RP11-575C20.2; DVS27-related protein; nuclear factor from high endothelial venules; chromosome 9 open reading frame 26 (NF-HEV); IL-1F11; IL-33; IL1F11; NFHEV; OTTHUMP00000021041; Interleukin-1 family member 11; Nuclear factor from high endothelial venules
• Gene ID: 90865
• mRNA Refseq: NM_033439
• Protein Refseq: NP_254274
• MIM: 608678
• UniProt ID: O95760
• Chromosome Location: 9p24
• Pathway: cytosolic DNA-sensing pathway; Influenza A
• Function: cytokine activity; protein binding