Recombinant Human IL1B protein, His-tagged

• Cat.No.: IL1B-460H
• Product Overview: Recombinant Human IL1B protein (Ala117-Ser269), fused to His-tag at the N-terminus, was expressed in 293F cell.

Specification

• Description: The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
• Source: HEK293
• Species: Human
• Tag: His
• Form: 20mM Tris, 150mM NaCl buffer (pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300）
• Molecular Mass: Predicted Molecular Mass: 19.0 kDa; Accurate Molecular Mass: 19 kDa
• Protein length: Ala117-Ser269
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in PBS or other buffer. Do not vortex.

Gene Information

• Gene Name: IL1B
• Official Symbol: IL1B
• Synonyms: IL-1; IL1F2; IL1beta; IL1-BETA
• Gene ID: 3553
• mRNA Refseq: NM_000576.3
• Protein Refseq: NP_000567.1
• MIM: 147720
• UniProt ID: P01584