Recombinant Human IGFBP5 protein

• Cat.No.: IGFBP5-5052H
• Product Overview: Recombinant Human IGFBP5 protein was expressed in Escherichia coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 252
• Description: An IGFBP5 gene was recently identified as being important for adaptation to varying water salinity in fish.
• Form: Lyophilized from a 0.2μm filtered concentrated solution in 10 mM Sodium Citrate, pH 3.0.
• Bio-activity: Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 cells is less than 0.4 μg/ml, corresponding to a specific activity of > 2500 IU/mg in the presence of 15 ng/ml of rHuIGF-II.
• Molecular Mass: Approximately 28.6 kDa, a single non-glycosylated polypeptide chain containing 252 amino acids.
• AA Sequence: LGSFVHCEPCDEKALSMCPPSPLGCELVKEPGCGCCMTCALAEGQSCGVYTERCAQGLRCLPRQDEEKPLHALLHGRGVCLNEKSYREQVKIERDSREHEEPTTSEMAEETYSPKIFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRIISAPEMRQESEQGPCRRHMEASLQELKASPRMVPRAVYLPNCDRKGFYKRKQCKPSRGRKRGICWCVDKYGMKLPGMEYVDGDFQCHTFDSSNVE
• Endotoxin: Less than 0.1 EU/μg of rHuIGF-BP5 as determined by LAL method.
• Purity: >96% by SDS-PAGE and HPLC analysis.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: IGFBP5
• Official Symbol: IGFBP5
• Synonyms: IGFBP5; insulin-like growth factor binding protein 5; insulin-like growth factor-binding protein 5; IBP-5; IGFBP-5; IGF-binding protein 5; IBP5
• Gene ID: 3488
• mRNA Refseq: NM_000599
• Protein Refseq: NP_000590
• MIM: 146734
• UniProt ID: P24593