Recombinant Human Insulin-like Growth Factor Binding Protein 5

• Cat.No.: IGFBP5-81H
• Product Overview: Recombinant Human IGFBP5 produced inE.Coliis a single, non-glycosylated, polypeptide chain containing 253 amino acids and having a molecular mass of 28613 Dalton. IGFBP5 is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: IGFBP5 is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Amino Acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Gly-Ser-Phe.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: IBP-5 was lyophilized from a concentrated (1mg/ml) solution containing 10 mM sodium Citrate PH 3.0.
• Solubility: It is recommended to reconstitute the lyophilized Insulin-Like Growth Factor Binding Protein-5 in sterile 18 MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Specific Activity: The ED₅₀, calculated by its ability to inhibit IGF-II induced proliferation of MCF-7 is < 0.3µg/ml in the presence of 15 ng/ml of Human IGF-II.
• Stability: Lyophilized IBP5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGFBP 5 should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: IGFBP5 insulin-like growth factor binding protein 5 [ Homo sapiens ]
• Synonyms: IBP-5; IBP5; IGFBP-5; IGFBP5; IGF-binding protein 5; insulin-like growth factor binding protein 5
• Gene ID: 3488
• mRNA Refseq: NM_000599
• Protein Refseq: NP_000590
• MIM: 146734
• UniProt ID: P24593
• Chromosome Location: 2q33-q36
• Pathway: Diabetes pathway
• Function: insulin-like growth factor I binding