Recombinant Human IGF2BP3 protein, His/T7-tagged

• Cat.No.: IGF2BP3-16H
• Product Overview: Recombinant Human IGF2BP3(Asn2~Ile343) fused with His/T7 tag at N-terminal was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Molecular Mass: 41.6 kDa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- NKLYIGNLS ENAAPSDLES IFKDAKIPVS GPFLVKTGYA FVDCPDESWA LKAIEALSGK IELHGKPIEV EHSVPKRQRI RKLQIRNIPP HLQWEVLDSL LVQYGVVESC EQVNTDSETA VVNVTYSSKD QARQALDKLN GFQLENFTLK VAYIPDEMAA QQNPLQQPRG RRGLGQRGSS RQGSPGSVSK QKPCDLPLRL LVPTQFVGAI IGKEGATIRN ITKQTQSKID VHRKENAGAA EKSITILSTP EGTSAACKSI LEIMHKEAQD IKFTEEIPLK ILAHNNFVGR LIGKEGRNLK KIEQDTDTKI TISPLQELTL YNPERTITVK GNVETCAKAE EEI
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Protein length: 2-343 a.a.

Gene Information

• Gene Name: IGF2BP3 insulin-like growth factor 2 mRNA binding protein 3 [ Homo sapiens ]
• Official Symbol: IGF2BP3
• Synonyms: IGF2BP3; insulin-like growth factor 2 mRNA binding protein 3; insulin-like growth factor 2 mRNA-binding protein 3; cancer/testis antigen 98; CT98; IGF II mRNA binding protein 3; IMP 3; hKOC; VICKZ family member 3; IGF2 mRNA-binding protein 3; IGF-II mRNA-binding protein 3; KH domain containing protein overexpressed in cancer; KH domain-containing protein overexpressed in cancer; IMP3; KOC1; IMP-3; VICKZ3; DKFZp686F1078
• Gene ID: 10643
• mRNA Refseq: NM_006547
• Protein Refseq: NP_006538
• MIM: 608259
• UniProt ID: O00425
• Chromosome Location: 7p15.3
• Pathway: Binding of RNA by Insulin-like Growth Factor-2 mRNA Binding Proteins (IGF2BPs/IMPs/VICKZs), organism-specific biosystem; Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem
• Function: RNA binding; mRNA 3-UTR binding; mRNA 5-UTR binding; nucleotide binding; protein binding; translation regulator activity