Recombinant Human IFI30 protein, His & T7-tagged

• Cat.No.: IFI30-1317H
• Product Overview: Recombinant Human IFI30 aa. (Met1~Lys250 (Accession # P13284) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 31.7kDa.
• Protein length: Met1~Lys250
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 4.8.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: IFI30 IFI30, lysosomal thiol reductase [ Homo sapiens (human) ]
• Official Symbol: IFI30
• Synonyms: IFI30; IFI30, lysosomal thiol reductase; GILT; IP30; IP-30; IFI-30; gamma-interferon-inducible lysosomal thiol reductase; gamma-interferon-inducible protein IP-30; interferon gamma-inducible protein 30 preproprotein; interferon, gamma-inducible protein 30; legumaturain
• Gene ID: 10437
• mRNA Refseq: NM_006332.4
• Protein Refseq: NP_006323.2
• UniProt ID: P13284