Recombinant Human IDH1 Protein, His-tagged

• Cat.No.: IDH1-022H
• Product Overview: Recombinant Human IDH1 protein with a C-terminal His-tag was expressed in E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
• Molecular Mass: 47.9 kDa
• Purity: ≥90% estimated by SDS-PAGE
• Unit Definition: One unit is defined as the amount of enzyme required to convert 1 µmol of NADP + to NADPH, using 1.5 mM isocitrate as a substrate, per minute at room temperature in 25 mM Tris-HCl, pH 7.5, 150 mM sodium chloride, and 5 mM MgCl2 .
• Stability: ≥ 1 year
• Storage: -80 centigrade
• Storage Buffer: 50 mM Tris-HCl, pH 7.5, containing 200 mM sodium chloride, 5 mM β-mercaptoethanol, 50 mM calcium chloride, and 20% glycerol
• Shipping: Dry ice in continental US, may vary elsewhere.

Gene Information

• Gene Name: IDH1 isocitrate dehydrogenase (NADP(+)) 1 [ Homo sapiens (human) ]
• Official Symbol: IDH1
• Synonyms: IDH1; isocitrate dehydrogenase (NADP(+)) 1; IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26; isocitrate dehydrogenase [NADP] cytoplasmic; NADP(+)-specific ICDH; NADP-dependent isocitrate dehydrogenase, cytosolic; NADP-dependent isocitrate dehydrogenase, peroxisomal; epididymis luminal protein 216; epididymis secretory protein Li 26; epididymis secretory sperm binding protein; isocitrate dehydrogenase (NADP(+)) 1, cytosolic; isocitrate dehydrogenase 1 (NADP+), soluble oxalosuccinate decarboxylase; EC 1.1.1.42
• Gene ID: 3417
• mRNA Refseq: NM_001282386
• Protein Refseq: NP_001269315
• MIM: 147700
• UniProt ID: O75874