Recombinant Human IARS protein, His & T7-tagged

• Cat.No.: IARS-8217H
• Product Overview: Recombinant Human IARS aa. (Met1~Arg500 (Accession # P41252)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Met1~Arg500
• Description: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Alternatively spliced transcript variants have been found.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 61.7kDa.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.6.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: IARS isoleucyl-tRNA synthetase [ Homo sapiens (human) ]
• Official Symbol: IARS
• Synonyms: IARS; isoleucyl-tRNA synthetase; IRS; ILRS; IARS1; ILERS; GRIDHH; PRO0785; isoleucine--tRNA ligase, cytoplasmic; isoleucine tRNA ligase 1, cytoplasmic
• Gene ID: 3376
• mRNA Refseq: NM_002161.5
• Protein Refseq: NP_002152.2
• UniProt ID: P41252