Recombinant Human Hypoxia Inducible Factor 1, Alpha Subunit Inhibitor

• Cat.No.: HIF1AN-5022H
• Product Overview: Recombinant human HIF1AN protein was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Cat. No.: HIF1AN-5022H
• Description: Hypoxia-inducible factor 1, alpha subunit inhibitor (HIF1AN), a member of the Fe2+ and 2-oxoglutarate-dependent dioxygenase superfamily, is a protein that hydoxylates a specific asparagine residue (Asn-803) within the HIF1alpha C-terminal transactivation domain. In normoxia, the HIF1AN-mediated hydroxylation of the HIF1alpha transactivation domain results in blockage of the HIF1alpha-p300/CBP interaction and represses transcriptional activity of HIF1alpha-targeted genes.
• Concentration: 1 mg/ml
• Source: Human
• Host: E.coli
• Form: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0.
• Purity: > 90% by SDS - PAGE
• Sequence: 1-349 amino acids: MAATAAEAVA SGSGEPREEA GALGPAWDES QLRSYSFPTR PIPRLSQSDP RAEELIENEE PVVLTDTNLV YPALKWDLEY LQENIGNGDF SVYSASTHKF LYYDEKKMAN FQNFKPRSNR EEMKFHEFVE KLQDIQQRGG EERLYLQQTL NDTVGRKIVM DFLGFNWNWI NKQQGKRGWG QLTSNLLLIG MEGNVTPAHY DEQQNFFAQI KGYKR
• Molecular Mass: 40.2 kDa (349 aa), confirmed by MALDI-TOF.
• Applications: SDS-PAGE
• Storage: Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
• Tag: Non

Gene Information

• Gene Name: HIF1AN hypoxia inducible factor 1, alpha subunit inhibitor [ Homo sapiens ]
• Official Symbol: HIF1AN
• Synonyms: HIF1AN; DKFZp762F1811; FIH1; FLJ20615; FLJ22027; hypoxia inducible factor 1, alpha subunit inhibitor; OTTHUMP00000020284; factor inhibiting HIF1; peptide-aspartate beta-dioxygenase; DKFZp762F1811; EC 1.14.11.16; FIH-1; Factor inhibiting HIF-1; Hypoxia-inducible factor asparagine hydroxylase; factor inhibiting HIF1; hypoxia-inducible factor 1, alpha subunit inhibitor
• Gene ID: 55662
• mRNA Refseq: NM_017902
• Protein Refseq: NP_060372
• MIM: 606615
• UniProt ID: Q9NWT6
• Chromosome Location: 10q24
• Pathway: HIF-2-alpha transcription factor network; Hypoxic and oxygen homeostasis regulation of HIF-1-alpha
• Function: iron ion binding; metal ion binding; oxidoreductase activity; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two; atoms of oxygen; peptide-aspartate beta-dioxygenase activity; protein binding