Recombinant Human HSP90AB1 293 Cell Lysate

• Cat.No.: HSP90AB1-5361HCL

Specification

• Description: Antigen standard for heat shock protein 90kDa alpha (cytosolic), class B member 1 (HSP90AB1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: HSP90AB1 heat shock protein 90kDa alpha (cytosolic), class B member 1 [ Homo sapiens ]
• Official Symbol: HSP90AB1
• Synonyms: HSP90AB1; heat shock protein 90kDa alpha (cytosolic), class B member 1; heat shock 90kD protein 1, beta , heat shock 90kDa protein 1, beta , HSPC2, HSPCB; heat shock protein HSP 90-beta; heat shock 84 kDa; heat shock protein beta; heat shock 90kD protein 1, beta; heat shock 90kDa protein 1, beta; 90-kda heat shock protein beta HSP90 beta; HSP84; HSPC2; HSPCB; D6S182; HSP90B; HSP90-BETA; FLJ26984
• Gene ID: 3326
• mRNA Refseq: NM_007355
• Protein Refseq: NP_031381
• MIM: 140572
• UniProt ID: P08238
• Chromosome Location: 6p12
• Pathway: Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Immune System, organism-specific biosystem; Inflammasomes, organism-specific biosystem; Innate Immune System, organism-specific biosystem
• Function: ATP binding; TPR domain binding; nitric-oxide synthase regulator activity; nucleotide binding; protein binding; protein serine/threonine kinase activity; unfolded protein binding