Recombinant Human HSF4 protein, His & GST-tagged

• Cat.No.: HSF4-5662H
• Product Overview: Recombinant Human HSF4 aa. (Val171~Arg284 (Accession # Q9ULV5)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: GST&His
• Protein Length: Val171~Arg284
• Description: Heat-shock transcription factors (HSFs) activate heat-shock response genes under conditions of heat or other stresses. HSF4 lacks the carboxyl-terminal hydrophobic repeat which is shared among all vertebrate HSFs and has been suggested to be involved in the negative regulation of DNA binding activity. Two alternatively spliced transcripts encoding distinct isoforms and possessing different transcriptional activity have been described.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 45.2kDa
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 8.3.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: HSF4 heat shock transcription factor 4 [ Homo sapiens (human) ]
• Official Symbol: HSF4
• Synonyms: HSF4; heat shock transcription factor 4; CTM; CTRCT5; heat shock factor protein 4; HSF 4; HSTF 4; hHSF4
• Gene ID: 3299
• mRNA Refseq: NM_001040667.2
• Protein Refseq: NP_001035757.1
• UniProt ID: Q9ULV5