Recombinant Human HSD17B2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: HSD17B2-1364H
• Product Overview: HSD17B2 MS Standard C13 and N15-labeled recombinant protein (NP_002144) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 42.8 kDa
• AA Sequence: MSTFFSDTAWICLAVPTVLCGTVFC KYKKSSGQLWSWMVCLAGLCAVCLL ILSPFWGLILFSVSCFLMYTYLSGQ ELLPVDQKAVLVTGGDCGLGHALCK YLDELGFTVFAGVLNENGPGAEELR RTCSPRLSVLQMDITKPVQIKDAYS KVAAMLQDRGLWAVINNAGVLGFPT DGELLLMTDYKQCMAVNFFGTVEVT KTFLPLLRKSKGRLVNVSSMGGGAP MERLASYGSSKAAVTMFSSVMRLEL SKWGIKVASIQPGGFLTNIAGTSDK WEKLEKDILDHLPAEVQEDYGQDYI LAQRNFLLLINSLASKDFSPVLRDI QHAILAKSPFAYYTPGKGAYLWICL AHYLPIGIYDYFAKRHFGQDKPMPR ALRMPNYKKKATTRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: HSD17B2 hydroxysteroid 17-beta dehydrogenase 2 [ Homo sapiens (human) ]
• Official Symbol: HSD17B2
• Synonyms: HSD17B2; hydroxysteroid (17-beta) dehydrogenase 2; estradiol 17-beta-dehydrogenase 2; HSD17; SDR9C2; short chain dehydrogenase/reductase family 9C; member 2; E2DH; 20-alpha-HSD; 17-beta-HSD 2; testosterone 17-beta-dehydrogenase; 20 alpha-hydroxysteroid dehydrogenase; 17-beta-hydroxysteroid dehydrogenase type 2; microsomal 17-beta-hydroxysteroid dehydrogenase; short chain dehydrogenase/reductase family 9C, member 2; EDH17B2
• Gene ID: 3294
• mRNA Refseq: NM_002153
• Protein Refseq: NP_002144
• MIM: 109685
• UniProt ID: P37059