Recombinant Human HSD11B1 Protein, His-tagged

• Cat.No.: HSD11B1-019H
• Product Overview: Recombinant Human HSD11B1 protein with a N-terminal His-tag was expressed in E.coli

Specification

• Description: The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Mutations in this gene and H6PD (hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase)) are the cause of cortisone reductase deficiency. Alternate splicing results in multiple transcript variants encoding the same protein.
• Source: E. coli
• Species: Human
• Tag: His
• Molecular Mass: 31.4 kDa
• Purity: ≥75% estimated by SDS-PAGE
• Unit Definition: One unit is defined as the amount of enzyme required to produce 1 nmol of cortisol per minute at 37 centigrade in 50 mM sodium phosphate buffer, pH 7.6, containing 100 mM sodium chloride, using 40 µM cortisone as substrate, and 500 µM NADPH.
• Stability: ≥ 6 months
• Storage: -80 centigrade
• Storage Buffer: 50 mM sodium phosphate buffer, pH 7.6, containing 100 mM sodium chloride and 20% glycerol
• Shipping: Dry ice in continental US, may vary elsewhere.

Gene Information

• Gene Name: HSD11B1 hydroxysteroid 11-beta dehydrogenase 1 [ Homo sapiens (human) ]
• Official Symbol: HSD11B1
• Synonyms: HSD11B1; hydroxysteroid 11-beta dehydrogenase 1; HDL; 11-DH; HSD11; HSD11B; HSD11L; CORTRD2; SDR26C1; 11-beta-HSD1; corticosteroid 11-beta-dehydrogenase isozyme 1; short chain dehydrogenase/reductase family 26C member 1; EC 1.1.1.146
• Gene ID: 3290
• mRNA Refseq: NM_001206741
• Protein Refseq: NP_001193670
• MIM: 600713
• UniProt ID: P28845