Recombinant Human HMOX1 protein, His & S-tagged

• Cat.No.: HMOX1-2604H
• Product Overview: Recombinant Human HMOX1 aa. (Leu61~Ile172 (Accession # P09601)) fused with N-terminal His & S tag was produced in E. coli cells.

Specification

• Description: Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.
• Source: E. coli
• Species: Human
• Tag: His&S
• Form: Freeze-dried powder
• Molecular Mass: 24kDa as determined by SDS-PAGE reducing conditions
• Protein length: Leu61~Ile172
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.7.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4

Gene Information

• Gene Name: HMOX1 heme oxygenase 1 [ Homo sapiens (human) ]
• Official Symbol: HMOX1
• Synonyms: HMOX1; heme oxygenase 1; HO-1; HSP32; HMOX1D; bK286B10; heme oxygenase 1; heat shock protein, 32-kD; heme oxygenase (decycling) 1
• Gene ID: 3162
• mRNA Refseq: NM_002133.2
• Protein Refseq: NP_002124.1
• UniProt ID: P09601