Recombinant Human HMGA1, His-tagged

• Cat.No.: HMGA1-3597H
• Product Overview: Recombinant human HMGA1 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-107 aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: High mobility group protein HMG-I/HMG-Y, also known as HMGA1, is a member of the non-histone chromosomal high mobility group protein (HMG) family. HMGA1 consists of a highly conserved AT-hook DNA-binding domain that mediates binding to AT-rich sequences in the minor groove of chromosomal DNA. It functions as architectural chromatin-binding transcription factor altering the conformation of DNA by modulating nuclear protein-DNA complexes. It is involved in many cellular processes including growth regulation, viral induction of beta-IFN gene and regulation of inducible gene transcription.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 50% glycerol, 0.2M NaCl
• Molecular Weight: 12.7 kDa (115aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
• Purity: > 90% by SDS - PAGE
• Concentration: 0.25 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MSESSSKSSQ PLASKQEKDG TEKRGRGRPR KQPPVSPGTA LVGSQKEPSE VPTPKRPRGR PKGSKNKGAA KTRKTTTTPG RKPRGRPKKL EKEEEEGISQ ESSEEEQLEH HHHHH
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: HMGA1 high mobility group AT-hook 1 [ Homo sapiens ]
• Official Symbol: HMGA1
• Synonyms: HMG-R; HMGIY; HMGA1A; MGC4242; MGC4854; MGC12816; HMGA1; high mobility group protein HMG-I/HMG-Y; HMG-I(Y); high mobility group protein R; high mobility group protein A1; high mobility group AT-hook protein 1; nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y; OTTHUMP00000016223; OTTHUMP00000016224; high-mobility group (nonhistone chromosomal) protein isoformsI and Y; OTTHUMP00000016222
• Gene ID: 3159
• mRNA Refseq: NM_002131
• Protein Refseq: NP_002122
• MIM: 600701
• UniProt ID: P17096
• Chromosome Location: 6p21
• Function: AT DNA binding; DNA binding; enzyme binding; ligand-dependent nuclear receptor transcription coactivator activity; peroxisome proliferator activated receptor binding; protein binding; retinoic acid receptor binding; retinoid X receptor binding; sequence-specific DNA binding transcription factor activity; transcription factor binding