Recombinant Human HLA-G protein(Gly25-Thr305(C66S)), His-Avi-tagged

• Cat.No.: HLA-G-502H
• Product Overview: Recombinant Human HLA-G protein(Gly25-Thr305(C66S))(P17693-1) is expressed from E.coli with His tag and Avi tag at the C-Terminus.

Specification

• Species: Human
• Source: E.coli
• Tag: Avi&His
• Protein Length: Gly25-Thr305(C66S)
• Form: Supplied as 0.22μm filtered solution in 20mM Tris, 500mM NaCl, 20% Glycerol (pH 8.0).
• Bio-activity: Immobilized Human HLA-G Free Heavy Chain, His Tag at 2μg/ml (100μl/Well) on the. Dose response curve for Anti-HLA-G Antibody, hFc Tag with the EC50 of 29.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 35.5 kDa. The protein migrates to 36-40 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
• Storage: Valid for 12 months from date of receipt when stored at -80°C.
  Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: HLA-G major histocompatibility complex, class I, G [ Homo sapiens ]
• Official Symbol: HLA-G
• Synonyms: HLA-G; major histocompatibility complex, class I, G; HLA G histocompatibility antigen, class I, G; HLA class I histocompatibility antigen, alpha chain G; b2 microglobulin; HLA G antigen; HLA class I molecule; MHC class I antigen G; HLA-G histocompatibility antigen, class I, G; MHC-G
• Gene ID: 3135
• mRNA Refseq: NM_002127
• Protein Refseq: NP_002118
• MIM: 142871
• UniProt ID: P17693