Active Recombinant Human HLA-G complex Protein (tetramer), His-Avi-tagged, PE-Labeled

• Cat.No.: HLA-G-505HP
• Product Overview: Recombinant PE-Labeled Human HLA-G Tetramer Protein(P17693-1(HLA-G)&P61769(B2M)&RIIPRHLQL)(Gly25-Thr305(HLA-G),Ile21-Met119(B2M) and RIIPRHLQL peptide) is expressed from Expi293 with His tag and Avi tag at the C-terminal,tetramer is assembled by biotinylated monomer and streptavidin.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Gly25-Thr305(HLA-G),Ile21-Met119(B2M) and RIIPRHLQL peptide
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.
• Bio-activity: Serial dilutions of Anti-LILRB2 Antibody were added into PE-Labeled Human HLA-G Tetramer, His Tag : Human LILRB2, mFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.45μg/ml.
• Molecular Mass: The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
• Storage: Valid for 12 months from date of receipt when stored at -80°C.
  Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized powder in sterile water to 100-200 ug/ml, and further dilute with other solvents. The dissolved protein can be stored at 4°C for 2-7 days and should be used up as soon as possible. If not in use for a short time, please store at -20°C.

Gene Information

• Gene Name: HLA-G major histocompatibility complex, class I, G [ Homo sapiens ]
• Official Symbol: HLA-G
• Synonyms: HLA-G; major histocompatibility complex, class I, G; HLA G histocompatibility antigen, class I, G; HLA class I histocompatibility antigen, alpha chain G; b2 microglobulin; HLA G antigen; HLA class I molecule; MHC class I antigen G; HLA-G histocompatibility antigen, class I, G; MHC-G
• Gene ID: 3135
• mRNA Refseq: NM_002127
• Protein Refseq: NP_002118
• MIM: 142871
• UniProt ID: P17693