Recombinant Human HLA-E protein, His-Avi-tagged

• Cat.No.: HLA-E-038H
• Product Overview: Recombinant Human HLA-E Complex Tetramer Protein(P61769&P13747&VMAPRTLVL) is expressed from HEK293 with His and Avi tag at the C-terminal. It contains Gly25-Ile305(HLA-E),Ile21-Met119(B2M) and VMAPRTLVL peptide.

Specification

• Description: HLA-E is a nonclassical member of the major histocompatibility complex class I gene locus. HLA-E protein shares a high level of homology with MHC Ia classical proteins: it has similar tertiary structure, associates with β2-microglobulin, and is able to present peptides to cytotoxic lymphocytes. The main function of HLA-E under normal conditions is to present peptides derived from the leader sequences of classical HLA class I proteins, thus serving for monitoring of expression of these molecules performed by cytotoxic lymphocytes.
• Source: HEK293
• Species: Human
• Tag: His&Avi
• Form: Supplied as 0.22um filtered solution in PBS (pH 7.4).
• Molecular Mass: The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein tetramer migrates to 230-260 kDa based on Tris-Bis PAGE result.
• Protein length: It contains Gly25-Ile305(HLA-E),Ile21-Met119(B2M) and VMAPRTLVL peptide.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
  > 95% as determined by SEC-HPLC
• Storage: The product should be stored at -70°C. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: HLA-E major histocompatibility complex, class I, E [ Homo sapiens (human) ]
• Official Symbol: HLA-E
• Synonyms: HLA class I histocompatibility antigen, alpha chain E; HLAE;sHLA-E
• Gene ID: 3133
• mRNA Refseq: NM_005516
• Protein Refseq: NP_005507
• MIM: 143010
• UniProt ID: P13747