Recombinant Human HLA-E complex Protein (tetramer), His-Avi-tagged, PE-Labeled

• Cat.No.: HLA-E-040HP
• Product Overview: Recombinant PE-Labeled Human HLA-E*01:03 Tetramer Protein(P13747(HLA-E*01:03)&P61769(B2M)&VMAPRTLVL)(Gly25-Ile305(HLA-E*01:03),Ile21-Met119(B2M) and VMAPRTLVL peptide) is expressed from Expi293 with His tag and Avi tag at the C-terminal,tetramer is assembled by biotinylated monomer and streptavidin.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Gly25-Ile305(HLA-E*01:03),Ile21-Met119(B2M) and VMAPRTLVL peptide
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.
• Molecular Mass: The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
• Storage: Valid for 12 months from date of receipt when stored at -80°C.
  Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized powder in sterile water to 100-200 ug/ml, and further dilute with other solvents. The dissolved protein can be stored at 4°C for 2-7 days and should be used up as soon as possible. If not in use for a short time, please store at -20°C.

Gene Information

• Gene Name: HLA-E major histocompatibility complex, class I, E [ Homo sapiens ]
• Official Symbol: HLA-E
• Synonyms: HLA-E; major histocompatibility complex, class I, E; HLA class I histocompatibility antigen, alpha chain E; MHC HLA-E alpha-1; lymphocyte antigen; MHC HLA-E alpha-2.1; MHC class I antigen E; HLA class I histocompatibility antigen, E alpha chain; MHC; QA1; EA1.2; EA2.1; HLA-6.2; DKFZp686P19218
• Gene ID: 3133
• mRNA Refseq: NM_005516
• Protein Refseq: NP_005507
• MIM: 143010
• UniProt ID: P13747