Recombinant Human HLA-DRB3, His-tagged

• Cat.No.: HLA-DRB3-13823H
• Product Overview: Recombinant Human HLA-DRB3 protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• ProteinLength: 31-228a.a.
• Description: HLA-DRB3 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB3 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.

Gene Information

• Gene Name: HLA-DRB3 major histocompatibility complex, class II, DR beta 3 [ Homo sapiens ]
• Official Symbol: HLA-DRB3
• Synonyms: major histocompatibility complex, class II, DR beta 3; 4951; MGC117330; major histocompatibility complex, class II, DR beta 3;DR7;MHC class II antigen DRB3;human leucocyte antigen DRB3;MHC class II HLA-DR beta 3 chain;MHC class II antigen DR beta 3 chain;HLA class II histocompatibility antigen, DR beta 3 chain;HLA class II histocompatibility antigen, DRB1-7 beta chain; HLA-DR3B
• Gene ID: 3125
• mRNA Refseq: NM_022555.3
• Protein Refseq: NP_072049.2
• MIM: 612735
• UniProt ID: P79483
• Chromosome Location: 6p21.3
• Pathway: Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem
• Function: MHC class II receptor activity; MHC class II receptor activity; peptide antigen binding; protein binding