Recombinant Human HLA-DMA, GST-tagged

• Cat.No.: HLA-DMA-13812H
• Product Overview: Recombinant Human HLA-DMA protein, fused to GST-tag, was expressed in E.coli and purified by GSH-sepharose.

Specification

• Description: HLA-DMA belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta chain (DMB), both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail.
• Source: E.coli
• Species: Human
• Tag: GST
• Protein length: 21-261a.a.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol.

Gene Information

• Gene Name: HLA-DMA major histocompatibility complex, class II, DM alpha [ Homo sapiens ]
• Official Symbol: HLA-DMA
• Synonyms: HLA-DMA; major histocompatibility complex, class II, DM alpha; HLA class II histocompatibility antigen, DM alpha chain; D6S222E; RING6; MHC class II antigen DMA; really interesting new gene 6 protein; class II histocompatibility antigen, M alpha chain; DMA; HLADM
• Gene ID: 3108
• mRNA Refseq: NM_006120
• Protein Refseq: NP_006111
• MIM: 142855
• UniProt ID: P28067
• Chromosome Location: 6p21.3
• Pathway: Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem

Reviews

08/17/2021

Their in-depth understanding of the protein's characteristics, applications, and potential limitations allows for valuable insights and recommendations, ensuring the success of my experiments.

06/20/2016

Its purity, integrity, and consistency ensure reliable and reproducible results, which are essential for meaningful scientific discoveries.

04/04/2016

Whether it's troubleshooting, protocol optimization, or general inquiries, their knowledgeable and responsive team is readily available to assist and solve any problems that may arise during the experimental process.

Q&As

How does HLA-DMA relate to autoimmune diseases?

Aberrant antigen presentation mediated by HLA-DMA has been implicated in autoimmune diseases, as it may lead to the presentation of self-antigens and the activation of autoreactive T cells.

How can HLA-DMA be a potential therapeutic target?

Modulating HLA-DMA activity could be explored as a therapeutic strategy in conditions where immune dysregulation occurs, such as autoimmune diseases, by influencing antigen presentation.

How does HLA-DMA contribute to the adaptive immune response?

HLA-DMA facilitates the loading of antigenic peptides onto MHC class II molecules, a process essential for the activation of CD4+ T cells and the adaptive immune response.

In transplantation, how does HLA-DMA impact graft acceptance or rejection?

Understanding HLA-DMA's role is crucial in transplantation, as it affects the presentation of antigens from the graft, influencing the immune response and the likelihood of graft acceptance or rejection.

Are there genetic variations in HLA-DMA that can influence disease susceptibility?

Genetic variations in the HLA-DMA gene may contribute to differences in antigen presentation and influence susceptibility to certain diseases, making it an area of interest in genetic studies.