Recombinant Human HLA 293 Cell Lysate

• Cat.No.: HLA-DMA-5499HCL

Specification

• Description: Antigen standard for major histocompatibility complex, class II, DM alpha (HLA-DMA) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: HLA-DMA major histocompatibility complex, class II, DM alpha [ Homo sapiens ]
• Official Symbol: HLA-DMA
• Synonyms: HLA-DMA; major histocompatibility complex, class II, DM alpha; HLA class II histocompatibility antigen, DM alpha chain; D6S222E; RING6; MHC class II antigen DMA; really interesting new gene 6 protein; class II histocompatibility antigen, M alpha chain; DMA; HLADM
• Gene ID: 3108
• mRNA Refseq: NM_006120
• Protein Refseq: NP_006111
• MIM: 142855
• UniProt ID: P28067
• Chromosome Location: 6p21.3
• Pathway: Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem

Reviews

08/17/2021

Their in-depth understanding of the protein's characteristics, applications, and potential limitations allows for valuable insights and recommendations, ensuring the success of my experiments.

06/20/2016

Its purity, integrity, and consistency ensure reliable and reproducible results, which are essential for meaningful scientific discoveries.

04/04/2016

Whether it's troubleshooting, protocol optimization, or general inquiries, their knowledgeable and responsive team is readily available to assist and solve any problems that may arise during the experimental process.

Q&As

How does HLA-DMA relate to autoimmune diseases?

Aberrant antigen presentation mediated by HLA-DMA has been implicated in autoimmune diseases, as it may lead to the presentation of self-antigens and the activation of autoreactive T cells.

How can HLA-DMA be a potential therapeutic target?

Modulating HLA-DMA activity could be explored as a therapeutic strategy in conditions where immune dysregulation occurs, such as autoimmune diseases, by influencing antigen presentation.

How does HLA-DMA contribute to the adaptive immune response?

HLA-DMA facilitates the loading of antigenic peptides onto MHC class II molecules, a process essential for the activation of CD4+ T cells and the adaptive immune response.

In transplantation, how does HLA-DMA impact graft acceptance or rejection?

Understanding HLA-DMA's role is crucial in transplantation, as it affects the presentation of antigens from the graft, influencing the immune response and the likelihood of graft acceptance or rejection.

Are there genetic variations in HLA-DMA that can influence disease susceptibility?

Genetic variations in the HLA-DMA gene may contribute to differences in antigen presentation and influence susceptibility to certain diseases, making it an area of interest in genetic studies.