Recombinant Human Histone Deacetylase 3, GST-tagged

• Cat.No.: HDAC3-1591H
• Product Overview: Full-length recombinant human HDAC3 was expressed by baculovirus in Sf9 insect cells using a C-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: HDAC3 or Histone deacetylase 3 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex that represses transcription when tethered to a promoter. HDAC3 may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. HDAC3 can also down-regulate p53 function and thus modulate cell growth and apoptosis.
• Applications: HDAC Assay; Western Blot
• Molecular Weight: 83 kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
• Purity: >70%
• Concentration: 0.1 ug/ul
• Specific Activity: 125 RLU/min/ng
• Sequences: Full length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Cell cycle; Diabetes pathways; Fatty acid, triacylglycerol; Metabolism of lipids and lipoproteins; Regulation of retinoblastoma protein; Retinoic acid receptors-mediated signaling; Signaling events mediated by HDAC Class I; Signaling events mediated by HDAC Class II; Signalling by NGF; Transcriptional Regulation of White Adipocyte Differentiation; Validated targets of C-MYC transcriptional repression; p75 NTR receptor-mediated signaling; p75NTR negatively regulates cell cycle via SC1

Gene Information

• Gene Name: HDAC3 histone deacetylase 3 [ Homo sapiens ]
• Official Symbol: HDAC3
• Synonyms: HDAC3; histone deacetylase 3; HD3; RPD3; RPD3-2; histone deacetylase 3; SMAP45; OTTHUMP00000160029
• Gene ID: 8841
• mRNA Refseq: NM_003883
• Protein Refseq: NP_003874
• MIM: 605166
• UniProt ID: O15379
• Chromosome Location: 5q31.1-q31
• Function: NAD-dependent histone deacetylase activity (H3-K14 specific); NAD-dependent histone deacetylase activity (H3-K9 specific); histone deacetylase activity; histone deacetylase activity (H3-K16 specific); histone deacetylase binding; hydrolase activity; protein binding; transcription factor binding; chromatin binding; DNA binding; protein deacetylase activity; transcription corepressor activity