Active Recombinant Human NCOR1, GST-tagged

• Cat.No.: NCOR1-86H
• Product Overview: Approximately 1:1 stoichiometric complex of recombinant human HDAC3 (histone deacetylase 3) with a GST fusion protein comprising the Deacetylase Activation Domain (DAD) of human NCOR1 (amino acids 397-503; SANT1 domain plus amino flanking residues). Produced by coexpression of HDAC3 with a GST-NCOR1(DAD) construct in an insect cell system, the complex displays ~100-fold higher deacetylase activity than isolated HDAC3. This HDAC3 preparation is active with various fluorogenic peptide substrates.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Protein Length: 397-503 a.a.
• Description: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.
• Form: Liquid. In 50mM TRIS, pH 8.0, 138 mM NaCl and 10% glycerol.
• Bio-activity: ≥150 U/µg. One U=1 pmol/min at 37°C, 100 µM, “Fluor de Lys™-SIRT1” Substrate (p53 K(Ac)-382 fluorogenic substrate.
• Molecular Mass: 49/40 kDa
• Purity: ≥90% (SDS-PAGE)
• Storage: -80°C

Gene Information

• Gene Name: NCOR1 nuclear receptor corepressor 1 [ Homo sapiens ]
• Official Symbol: NCOR1
• Synonyms: NCOR1; nuclear receptor corepressor 1; nuclear receptor co repressor 1; hCIT529I10; hN CoR; KIAA1047; MGC104216; N CoR; thyroid hormone and retinoic acid receptor associated corepressor 1; TRAC1; nuclear receptor co-repressor 1; thyroid hormone- and retinoic acid receptor-associated corepressor 1; N-CoR; N-CoR1; hN-CoR
• Gene ID: 9611
• mRNA Refseq: NM_006311
• Protein Refseq: NP_006302
• MIM: 600849
• UniProt ID: O75376
• Chromosome Location: 17p11.2
• Pathway: Adipogenesis, organism-specific biosystem; BMAL1:CLOCK/NPAS2 Activates Circadian Expression, organism-specific biosystem; C-MYB transcription factor network, organism-specific biosystem; Circadian Clock, organism-specific biosystem; Circadian Repression of Expression by REV-ERBA, organism-specific biosystem; Delta-Notch Signaling Pathway, organism-specific biosystem; Developmental Biology, organism-specific biosystem
• Function: histone deacetylase binding; histone deacetylase regulator activity; protein binding; retinoid X receptor binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; thyroid hormone receptor binding; transcription corepressor activity; transcription corepressor activity; transcription regulatory region DNA binding