Recombinant Human HINT1 Protein (M1-G126), Tag Free

• Cat.No.: HINT1-0472H
• Product Overview: Recombinant Human PKCi (M1-G126) (H114A) Protein was expressed in E. coli.

Specification

• Description: Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2. Hydrolyzes adenosine 5'monophosphomorpholidate (AMP-morpholidate) and guanosine 5'monophosphomorpholidate (GMP-morpholidate). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His-AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester. Hydrolyzes 3-indolepropionic acyl-adenylate, tryptamine adenosine phosphoramidate monoester and other fluorogenic purine nucleoside tryptamine phosphoramidates in vitro. Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O-phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide. In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1. Modulates p53/TP53 levels and p53/TP53-mediated apoptosis. Modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin-protein ligase complex. Also exhibits SUMO-specific isopeptidase activity, deconjugating SUMO1 from RGS17. Deconjugates SUMO1 from RANGAP1.
• Source: E. coli
• Species: Human
• Tag: Non
• Form: Liquid
• Protein length: M1-G126
• Endotoxin: < 0.01 EU per μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: HINT1 histidine triad nucleotide binding protein 1 [ Homo sapiens (human) ]
• Official Symbol: HINT1
• Synonyms: HINT1; histidine triad nucleotide binding protein 1; HINT, histidine triad nucleotide binding protein, PRKCNH1; histidine triad nucleotide-binding protein 1; PKCI 1; protein kinase C inhibitor 1; adenosine 5-monophosphoramidase; protein kinase C-interacting protein 1; HINT; PKCI-1; PRKCNH1; FLJ30414; FLJ32340
• Gene ID: 3094
• mRNA Refseq: NM_005340
• Protein Refseq: NP_005331
• MIM: 601314
• UniProt ID: P49773