Recombinant Human HGS Protein, His-tagged

• Cat.No.: HGS-2238H
• Product Overview: Recombinant Human HGS Protein (H+Glu468+Gln741+LESENSNSSSVDKLAAALEHHHHHH) with a N-His tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene regulates endosomal sorting and plays a critical role in the recycling and degradation of membrane receptors. The encoded protein sorts monoubiquitinated membrane proteins into the multivesicular body, targeting these proteins for lysosome-dependent degradation.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 36.4 kDa
  Accurate Molecular Mass: 40 kDa
• Protein length: H+Glu468+Gln741+LESENSNSSSVDKLAAALEHHHHHH
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: HGS hepatocyte growth factor-regulated tyrosine kinase substrate [ Homo sapiens (human) ]
• Official Symbol: HGS
• Synonyms: HGS; hepatocyte growth factor-regulated tyrosine kinase substrate; Hrs; Vps27; ZFYVE8; protein pp110; human growth factor-regulated tyrosine kinase substrate; HRS; FLJ11421; FLJ39878
• Gene ID: 9146
• mRNA Refseq: NM_004712
• Protein Refseq: NP_004703
• MIM: 604375
• UniProt ID: O14964

Reviews

05/13/2019

Minimal background noise in our assays was indicative of the product's superior quality.

01/24/2018

Compatibility with a range of assays expanded our research possibilities significantly.

12/19/2016

Our research benefited greatly from the product's ability to induce specific cellular responses, enriching our findings.

Q&As

How does HGS contribute to the regulation of cell signaling and receptor downregulation in the endocytic pathway?

HGS contributes to the regulation of cell signaling by promoting the downregulation of activated receptors in the endocytic pathway.

What diseases or conditions are associated with dysregulation or abnormal expression of HGS?

Dysregulation or abnormal expression of HGS is associated with conditions such as cancer and neurodegenerative disorders.

How does HGS participate in the endocytic pathway, particularly in the degradation of receptor tyrosine kinases (RTKs)?

HGS participates in the endocytic pathway by facilitating the degradation of receptor tyrosine kinases (RTKs) through the ESCRT complex.

What is the primary function of Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS) in cellular processes and intracellular trafficking?

Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS) functions in cellular processes, primarily playing a role in intracellular trafficking.

In which cellular compartments is HGS predominantly localized?

HGS is predominantly localized in endosomes and lysosomes, key compartments for the endocytic pathway.

Are there specific interactions or binding partners of HGS that influence its role in intracellular trafficking?

Specific interactions or binding partners of HGS include ESCRT components, influencing its role in receptor downregulation and intracellular trafficking.

Are there therapeutic implications or potential targets related to HGS in diseases involving intracellular trafficking and signaling?

There are potential therapeutic implications related to HGS in diseases involving intracellular trafficking and signaling, making it a target for drug development.