Recombinant Human HDGF Protein, His-tagged

• Cat.No.: HDGF-159H
• Product Overview: Recombinant Human Hepatoma-Derived Growth Factor is produced by our E.coli expression system and the target gene encoding Met1-Tyr100 is expressed with a 6His tag at the C-terminus.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-100 a.a.
• Description: Hepatoma-Derived Growth Factor is a original member of the HDGF family. HDGF is a cytoplasmic protein and contains one PWWP domain. HDGF expression levels are high in the nucleus and cytoplasm of smooth muscle and endothelial cells. HDGF has proliferative, angiogenic and neurotrophic activity. HDGF was initially characterized as a secreted mitogen from the Huh-7 human hepatoma cell line. As a heparin-binding protein, which is highly expressed in tumor cells where it stimulates proliferation. HDGF has mitogenic activity for fibroblasts and acts as a transcriptional repressor. It has been shown that HDGF is linked with tumorigenesis and the growth of cancer.
• Form: Lyophilized from a 0.2 um filtered solution of 20mM Tris, 1mM DTT, 1mM EDTA, pH 7.5.
• Molecular Mass: Predicted Molecular Weight: 12.6kDa
  Apparent Molecular Weight: 14kDa, reducing conditions.
• AA Sequence: Met1-Tyr100
• Endotoxin: Less than 0.1 ng/ug (1 EU/ug).
• Purity: >95%
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
  Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months.
• Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
  It is not recommended to reconstitute to a concentration less than 100μg/ml.
  Dissolve the lyophilized protein in distilled water.
  Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Quality Statement: Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Shipping: The product is shipped at ambient temperature.
  Upon receipt, store it immediately at the temperature listed below.

Gene Information

• Gene Name: HDGF heparin binding growth factor [ Homo sapiens (human) ]
• Official Symbol: HDGF
• Synonyms: Hepatoma-Derived Growth Factor; High Mobility Group Protein 1-Kike 2; HMG-1L2; HMG1L2
• Gene ID: 3068
• mRNA Refseq: NM_001126050.2
• Protein Refseq: NP_001119522.1
• MIM: 600339
• UniProt ID: P51858