Recombinant Human HDGF 293 Cell Lysate

• Cat.No.: HDGF-5598HCL

Specification

• Description: Antigen standard for hepatoma-derived growth factor (high-mobility group protein 1-like) (HDGF), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: HDGF hepatoma-derived growth factor [ Homo sapiens ]
• Official Symbol: HDGF
• Synonyms: HDGF; hepatoma-derived growth factor; hepatoma derived growth factor (high mobility group protein 1 like); high mobility group protein 1 like; HMG1L2; HMG-1L2; high mobility group protein 1-like 2; FLJ96580; DKFZp686J1764
• Gene ID: 3068
• mRNA Refseq: NM_001126050
• Protein Refseq: NP_001119522
• MIM: 600339
• UniProt ID: P51858
• Chromosome Location: 1q21-q23
• Pathway: Activation of Chaperone Genes by XBP1(S), organism-specific biosystem; Activation of Chaperones by IRE1alpha, organism-specific biosystem; Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem; Unfolded Protein Response, organism-specific biosystem
• Function: DNA binding; growth factor activity; heparin binding; nucleotide binding

Reviews

03/30/2021

It demonstrates excellent performance in ELISA, consistently delivering accurate and reliable results.

06/25/2020

I highly recommend the HDGF protein for a variety of research applications.

01/03/2020

The HDGF protein has proven to be an invaluable tool in protein electron microscopy structure analysis.

Q&As

Are there any side effects associated with HDGF-targeted therapies?

The potential side effects of HDGF-targeted therapies are still under investigation, and research aims to minimize any adverse effects.

Can HDGF-targeted therapies be personalized for individual patients?

Personalized HDGF-targeted therapies are an area of research interest, with efforts to identify patient-specific variations in HDGF expression.

How does HDGF impact cardiovascular health?

HDGF has been implicated in cardiovascular diseases, where it plays a role in angiogenesis and tissue repair, making it a potential therapeutic target for heart-related conditions.

Is HDGF involved in tissue regeneration?

Yes, HDGF is involved in tissue regeneration by promoting cell growth and supporting the repair of damaged tissues.

How does HDGF interact with other proteins in cellular processes?

HDGF interacts with various proteins involved in cell signaling, growth, and survival pathways, influencing cellular processes.