Recombinant Human HBA1 protein, His-tagged

• Cat.No.: HBA1-7859H
• Product Overview: Recombinant Human HBA1 aa. (Met1~Arg142 (Accession # P69905)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 16.8kDa
• Protein length: Met1~Arg142
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 8.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: HBA1 hemoglobin subunit alpha 1 [ Homo sapiens (human) ]
• Official Symbol: HBA1
• Synonyms: HBA1; hemoglobin subunit alpha 1; HBH; HBA-T3; hemoglobin subunit alpha; alpha one globin; alpha-2 globin chain; delta globin; hemoglobin alpha 1 globin chain; hemoglobin, alpha 1
• Gene ID: 3039
• mRNA Refseq: NM_000558.4
• Protein Refseq: NP_000549.1
• UniProt ID: P69905