Recombinant Human HAMP protein, His & Fc-tagged

• Cat.No.: HAMP-458H
• Product Overview: Recombinant Human HAMP (P81172) aa. (Ser25~Thr84) fused with His & Fc tag was produced in HEK293F cells.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: Ser25~Thr84
• Description: The product encoded by this gene is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity against bacteria and fungi. Mutations in this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that results in cardiomyopathy, cirrhosis, and endocrine failure.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 34.1kDa; Accurate Molecular Mass: 33&40kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 95%
• Characteristic: Isoelectric point is 9.4.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays; Purification; Amine Reactive Labeling
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200ug/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: HAMP hepcidin antimicrobial peptide [ Homo sapiens (human) ]
• Official Symbol: HAMP
• Synonyms: HAMP; hepcidin antimicrobial peptide; HEPC; PLTR; HFE2B; LEAP1; hepcidin; liver-expressed antimicrobial peptide 1; putative liver tumor regressor
• Gene ID: 57817
• mRNA Refseq: NM_021175.3
• Protein Refseq: NP_066998.1
• UniProt ID: P81172