Recombinant Dog HAMP Protein, His&GST-tagged

• Cat.No.: HAMP-2732D
• Product Overview: Recombinant Dog HAMP Protein (Val23-Thr85) with N-His&GST tag was expressed in E. coli.

Specification

• Species: Dog
• Source: E.coli
• Tag: GST&His
• Protein Length: Val23-Thr85
• Description: Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin/SLC40A1, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 36.9 kDa
  Accurate Molecular Mass: 42 kDa
• Purity: > 80%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: HAMP hepcidin antimicrobial peptide [ Canis lupus familiaris (dog) ]
• Official Symbol: HAMP
• Synonyms: HAMP; hepcidin antimicrobial peptide; hepcidin; HFE2B; PLTR; LEAP1; Liver-expressed antimicrobial peptide 1; Putative liver tumor regressor
• Gene ID: 492281
• mRNA Refseq: NM_001007140
• Protein Refseq: NP_001007141
• UniProt ID: Q5U9D2