Recombinant Human Gutathione Proxidase 2 (gastrointestinal), His-tagged

• Cat.No.: GPX2-1325H
• Product Overview: GPX2(u40C), 1-190aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-190 a.a.
• Description: GPX2, also known as glutathione peroxidase 2, belongs to the glutathione peroxidase family, consisting of eight known glutathione peroxidases (Gpx1-8) in humans. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. GPX2 could play a major role in protecting mammals from the toxicity of ingested organic hydroperoxides. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. Recombinant human GPX2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
• Form: Liquid
• Molecular Mass: 24.1 kDa (210aa), confirmed by MALDI-TOF
• AA Sequence: 1-190aa
• Purity: >95% by SDS-PAGE.
• Storage: Can be stored at 4oC short term (1-2 weeks). For long term storage, aliquot and store at -20oC or - 70oC. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)
• Storage Buffer: 20mM Tris-HCl buffer (pH7.5) containing 40% glycerol, 0.15M NaCl, 1mM DTT

Gene Information

• Gene Name: GPX2 glutathione peroxidase 2 (gastrointestinal) [ Homo sapiens ]
• Official Symbol: GPX2
• Synonyms: GPX2; glutathione peroxidase 2 (gastrointestinal); glutathione peroxidase 2; GSHPX GI; glutathione peroxidase-gastrointestinal; glutathione peroxidase-related protein 2; gastrointestinal glutathione peroxidase 2; GPRP; GPx-2; GI-GPx; GPRP-2; GPx-GI; GSHPx-2; GSHPX-GI
• Gene ID: 2877
• mRNA Refseq: NM_002083
• Protein Refseq: NP_002074
• MIM: 138319
• UniProt ID: P18283
• Chromosome Location: 14q24.1
• Pathway: Arachidonic acid metabolism, organism-specific biosystem; Arachidonic acid metabolism, conserved biosystem; Folate Metabolism, organism-specific biosystem; Glutathione metabolism, organism-specific biosystem; Glutathione metabolism, organism-specific biosystem; Glutathione metabolism, conserved biosystem; Selenium Metabolism and Selenoproteins, organism-specific biosystem
• Function: electron carrier activity; glutathione peroxidase activity; oxidoreductase activity