Recombinant Human GUCY1A3 293 Cell Lysate

• Cat.No.: GUCY1A3-5677HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for guanylate cyclase 1, soluble, alpha 3 (GUCY1A3), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC206063, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: GUCY1A3 guanylate cyclase 1, soluble, alpha 3 [ Homo sapiens ]
• Official Symbol: GUCY1A3
• Synonyms: GUCY1A3; guanylate cyclase 1, soluble, alpha 3; GUC1A3; guanylate cyclase soluble subunit alpha-3; GC SA3; GCS-alpha-1; GCS-alpha-3; GC-S-alpha-1; soluble guanylate cyclase large subunit; GUCA3; GC-SA3; GUCSA3; GUCY1A1
• Gene ID: 2982
• mRNA Refseq: NM_000856
• Protein Refseq: NP_000847
• MIM: 139396
• UniProt ID: Q02108
• Chromosome Location: 4q31.3-q33
• Pathway: Gap junction, organism-specific biosystem; Gap junction, conserved biosystem; Hemostasis, organism-specific biosystem; Long-term depression, organism-specific biosystem; Long-term depression, conserved biosystem; Myometrial Relaxation and Contraction Pathways, organism-specific biosystem; Nitric oxide stimulates guanylate cyclase, organism-specific biosystem
• Function: GTP binding; guanylate cyclase activity; heme binding; nucleotide binding; receptor activity