Recombinant Human GTF2H3 293 Cell Lysate

• Cat.No.: GTF2H3-5695HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for general transcription factor IIH, polypeptide 3, 34kDa (GTF2H3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: GTF2H3 general transcription factor IIH, polypeptide 3, 34kDa [ Homo sapiens ]
• Official Symbol: GTF2H3
• Synonyms: GTF2H3; general transcription factor IIH, polypeptide 3, 34kDa; general transcription factor IIH, polypeptide 3 (34kD subunit); general transcription factor IIH subunit 3; BTF2; TFIIH; BTF2 p34; basic transcription factor 2 34 kDa subunit; general transcription factor IIH polypeptide 3; TFIIH basal transcription factor complex p34 subunit; TFB4
• Gene ID: 2967
• mRNA Refseq: NM_001516
• Protein Refseq: NP_001507
• MIM: 601750
• UniProt ID: Q13889
• Chromosome Location: 12q24.31
• Pathway: Basal transcription factors, organism-specific biosystem; Basal transcription factors, conserved biosystem; DNA Repair, organism-specific biosystem; Disease, organism-specific biosystem; Dual incision reaction in GG-NER, organism-specific biosystem; Dual incision reaction in TC-NER, organism-specific biosystem; Eukaryotic Transcription Initiation, organism-specific biosystem
• Function: contributes_to DNA-dependent ATPase activity; contributes_to RNA polymerase II carboxy-terminal domain kinase activity; damaged DNA binding; metal ion binding; protein N-terminus binding; contributes_to protein kinase activity; sequence-specific DNA binding transcription factor activity; translation factor activity, nucleic acid binding